Project

Part:BBa_K3113203:Design

Designed by: Alejandro Salinas Illarena, Joshua Hesse   Group: iGEM19_Munich   (2019-09-05)


pCAG_fluc_C/Dbox


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutations were performed to remove illegal restriction sites. The fusion protein was designed to function in mammalian cells.


Source

The sequence of fluc originates in Photinus pyralis and the sequence of the C/D-Box originates in Archaeoglobus fulgidus.


References